I am a Research Fellow at Ifremer (“Institut Français de Recherche pour l’Exploitation de la Mer” – French Research Institute for Exploitation of the Sea), France. As ecophysiologist my research interests span on integrative approaches from molecules to the whole-organism to investigate how physiological mechanisms underlie life-history trajectories in animals. Specifically, I am examining whether energy metabolism and oxidative stress are proximate causes of life history variation and underlie trade-offs. I also focus on the importance of mitochondrial plasticity in responses to environmental change. My research is pushing the boundary of ecophysiological research by combining multidisciplinary cutting-edge approaches and technological breakthroughs.
We had a very nice time, and succesful experiment on the effects of temperature and oxygen availability in vitro on the mitochondrial efficiency of red muscle of Sea bass with Elisa Thoral from the Laboratoire d’Ecologie des Hydrosystèmes Naturels et Anthropisés, Lyon, France.
©Ifremer Stephane Lesbats
PhD position in Ecophysiology of Marine Fish at the Laboratory of Marine Sciences, Ifremer – Along with Marie Vagner, Fabienne Legrand, Philippe Soudant, José Zambonino, Christos Chinopoulos, I am currently seeking motivated applicants for our PhD position to examine the mitochondrial responses to global change in marine fish.
Application deadline: 27 May; Starting date: 1st October 2019. For more info, click here!
Join me at the Sociey for Experimental Biology’s Annual Meeting in a session on “From genes to behaviour: Acclimation mechanisms with potential use as biomarkers in disturbed coastal habitats“. in Seville (2-5 July 2019). Abstract deadline: 15 March 2019.
About 20 secondary school students have spent 3 days at Ifremer; they have met colleagues that have different jobs. I enjoyed being the tutor of a few of them.
Science publishes Careers articles showcasing the life of researchers. I had the pleasure of “contributing” to the latest article by being interviewed for an article entitled “Considering going abroad for work? Recent research can help you weigh the pros and cons”. Check it out here.
For those interested in Evo-Eco-Physiology, an intro to a serie of papers that address a few key challenges in mitochondrial research: in what ways can mitochondria shape animal performance.
Wendy R Hood, Steven N Austad, Pierre Bize, Ana Gabriela Jimenez, Kristi L Montooth, Patricia M Schulte, Graham R Scott, Inna Sokolova, Jason R Treberg, Karine Salin. The Mitochondrial Contribution to Animal Performance, Adaptation, and Life-History Variation, Integrative and Comparative Biology (PDF on request).
Earlier this month several members of the lab organized the first international conference on Lipids in Ocean. It was an incredibly fun conference packed with amazing and inspiring talks; hopefully the first of many.
End of a many month experiment to investigate the “Temporal repeatability of inter-individual variation in mitochondrial function in red blood cells of Sea bass“; with Léna Jégo and Sophie Collet.
Summer school: in this event we (myself and colleagues within the lab) presented our latest work to a very enthusiastic group of secondary school teachers. Using a PowerPoint presentation as a starting point, the resulting discussions were excellent and cover a broad range of topics including global change, nutrition, human health and what it’s like to be a marine biologist.
Poster presentation at the SEB 2018, Florence, Italy: Depletion in dietary Omega 3 and mitochondrial efficiency in mullet.
Publication of our study on “How metabolic adjustments in response to food scarcity can have long-term negative effects“.
Salin K., Villasevil E.M., Anderson G.J., Auer, S.K., Selman, C., Hartley, R.C., Mullen, W., Chinopoulos, C. and Metcalfe, N.B. Decreased mitochondrial metabolic requirements in fasting animals carry an oxidative cost. Functional Ecolology. 2018;00:1–9. (doi:10.1111/1365-2435.13125) PDF
Start of a couple of months experiment to investigate the effect of depletion in dietary Omega 3 on mitochondrial efficiency in golden grey mullet Liza aurata; in collaboration with Marie Vagner, at LIttoral, ENvironment and Societies (LIENSs) – UMR 7266, La Rochelle University, France.
Introducing the high-resolution respirometry of the red blood cell mitochondria of Sea bass Dicentrarchus labrax to Sarah Howald, Louise Cominassi and Carmen Gonzalel Fernandez for tweaking the protocol of the cell permeabilization. The protocol includes steps of washing of the blood that has been freshly collected on an anesthetized fish, plus permeabilization of the red blood cells based on a protocol developed by Damien Roussel (Université Lyon 1, France) and Tony Hickey (University of Auckland, NZ). This assay will be used in our laboratory to determine how fish mitochondria respond to environmental changes.
Join us in Florence for Mitochondria in changing climates: Biosensors and mediators of animal resilience – Submit your abstract for the Society of Experimental Biology’s Annual Meeting – Deadline: 30 March
Salon Azimut Brest: Interactive time at a career choice event in Brest. Several staff members of Ifremer have been presenting their job such as IT service, admin or research in marine sciences. It was impressive to see so many motivated and ambitious students.
SICB 2018 San Francisco: We had a very nice conference!! The symposium “Inside the black box: the mitochondrial basis of life-history variation and animal performance” was a fantastic, well-attended session with mutidisplinary talks spanning all major animal taxa. Thanks to the speakers, Inna Sokolova, Jay Treberg, Ana Jimenez, Steven Austad, Pierre Bize, Kristi Montooth, Graham Scott and Trish Schulte for their great talk; and Wendy Hood for the helping in the organization.
Frank Seebacher and I are organising a session on ‘Mitochondria in changing climates: Biosensors and mediators of animal resilience’ at the Society of Experimental Biology’s Annual Meeting in Florence (3-6 July 2018). This is an exciting meeting to take part in with over 800 delegates from around the world expected to attend and a very diverse programme.
Abstract submission for the meeting is now open and we wanted to ask you to consider submitting your research to our session.
Start of a tenure position: Research Fellow at IFREMER (“Institut Français de Recherche pour l’Exploitation de la Mer” – French Research Institute for Exploitation of the Sea). I am in the Laboratory of Adaptation, Reproduction and Nutrition, which is part of the Laboratory of Environmental Marine Sciences.
End of the post-doc life! But also emotional ending of 4 years being part of the institute of Biodiversity, Animal Health and Comparative Medicine at the University of Glasgow, where I was working on a project run by Neil Metcalfe. The project also involves Sonya Auer, Graeme Anderson, Julie Nati, Elettra Leo and Darryl McLennan.
Start of an experiment to investigate the effects of dietary content in antioxidant and daily fluctuations of the thermal environment on oxidative stress in fish; with Elettra Leo, and in collaboration with Matt Slater, Alfred Wegener Institute, Germany.
Welcome Julie Nati who will determine the reaction norms of the mitochondrial oxidative capacities of minnow (Phoxinus phoxinus) in response to two co-occurring environmental stressors: elevated temperature and hypoxia; in collaboration with Tommy Norin.
It is our pleasure to invite you to attend the symposium ‘Inside the black box: the mitochondrial basis of life-history variation and animal performance’ which will be held at the Society for Integrative and Comparative Biology meeting Jan 3-7, 2018 in San Francisco, CA.
In addition to the main symposium, companion oral and poster sessions will also be held. You can opt to participate in the companion oral or poster session when submitting the abstract online. Deadline for submissions: September 6, 2017.
Great evening at the Café Scientifique for the Glasgow Science Festival. A lot of interesting discussions and a great interest for the mitochondria model (made of a potato and play doh).
Following the publication of the methodology to determine the effective P/O ratio, we now supply supplementary materials to extract and process the raw oxygen and fluorescence signals obtained from an O2k oxygraph equipped with an LED Module, with the steps required to complete the Excel template file as elaborated in Salin et al. (2016, Physio Rep).
I am officially a STEM Ambassador!
I have been awarded an Early Career Mobility Scheme from the University of Glasgow (£5k). This award allows me to visit (for 2 months) Frank Seebacher‘s lab in the University of Sydney where we will experimentally manipulate the efficiency with which the mitochondria transform energy to estimate the impact of mitochondrial efficiency on animal locomotor performance.
Oral presentation at the SICB 2017, New Orleans, LA: How Does Mitochondrial Functioning Constrain Energy Efficiency?
Acceptance of our method article on an assay that measures ROS in living animals and offers the possibility to store samples prior to analysis, so finally overcoming the main limitations of in vitro assays. We provide a detailed description of how to develop the MitoB protocol in organisms for which the method has never been applied before, with a general focus on issues that are likely to face ecologists and evolutionary biologists working on oxidative stress.
Salin, K., Auer, S. K., Villasevil, E. M., Anderson, G. J., Cairns, A. G., Mullen, W., Hartley, R. C., and Metcalfe, N. B.(2017) Using the MitoB method to assess levels of reactive oxygen species in ecological studies of oxidative stress.Scientific Reports, 7, 41228. (doi: 10.1038/srep41228) PDF
I was delighted to be part of the Women of the Future Awards in London, with so many inspirational women. I have been nominated for this UK award as one of 5 dynamic young females in the Science section.
Start of a 3-month experiment to investigate the link between mitochondrial phenotype and protein synthesis; in collaboration with Simon Lamarre, Université de Moncton, Canada.
Acceptance of our article on a new method that simultaneously quantifies both ATP production and oxygen consumption in tissue homogenates, so for the first time allowing the calculation of P/O ratios from tissue samples as small as tens of milligrams; in collaboration with Christos Chinopoulos, Semmelweis University, Hungary.
Salin, K., Villasevil, E., Auer, S., Anderson, G., Selman, C., Metcalfe, N., and Chinopoulos, C. (2016) Simultaneous measurement of mitochondrial respiration and ATP production in tissue homogenates and calculation of effective P/O ratios. Physiological Reports, 4(20), e13007. (doi:10.14814/phy2.13007) PDF
Our project would be an exciting blend of experimental biology and modelling to examine the flexible nature of leadership in animal social groups spaOur project would be an exciting blend of experimental biology and modelling to examine the flexible nature of leadership in animal social groups spanhttps://wwz.ifremer.fr/en/